Journal: PLoS ONE

Article Title: The p21-Activated Kinase (PAK) Family Member PakD Is Required for Chemorepulsion and Proliferation Inhibition by Autocrine Signals in Dictyostelium discoideum

doi: 10.1371/journal.pone.0096633

Figure Lengend Snippet: ( A ) act15::PakD-GFP cells were grown in glass chamber slides overnight, then fixed and stained with DAPI (blue), imaged by fluorescence microscopy using a 100× objective, and processed using Autodeblur deconvolution software (Bitplane software, Zurich, Switzerland). PakD-GFP signal (green) is shown superimposed on a transmitted light image. Bar is 10 µm. ( B ) Live pakD – /act15::PakD-GFP cells grown as a colony in glass chamber slides were imaged by fluorescence microscopy. The imaged cell is moving upwards, as seen by time-lapse microscopy. The arrow indicates PakD-GFP at the rear of the cell. A single punctum structure is also visible. The cell border is shown with a dashed line. Bar is 10 µm. ( C ) PakD-GFP (green) does not colocalize with either the cell nucleus (blue) or with staining for an antibody against ddCP224 (Red), a marker for the centrosome. Bar is 5 µm.

Article Snippet: Z-stacks were then processed using Autodeblur deconvolution software (Bitplane software, Zurich, Switzerland).

Techniques: Staining, Fluorescence, Microscopy, Software, Time-lapse Microscopy, Marker